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KMID : 0360319950270030360
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Journal of Korean Cancer Research Association
1995 Volume.27 No. 3 p.360 ~ p.373
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The Effect of Laminin and Type IV Collagen on the Adhesion and Invasion of Human Gastrocytes and Gastric Cancer Cells In Vitro
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김병식
김진천/노선애/박건춘/최국진
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Abstract
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Extracellular matrix is important in the metastasis of tumor, and basement membrane acts not only as tissue barrier but also as adhesive substrate for tumor cell invasion. Laminin and type IV collagen are major components of basement membrane and
important in tumor invasion. Adhesion and invasion studies about metastasis of the tumor have been performed mainly in colon cancer or breast cancer but rare in gastric cancer. The purpose of this study was to investigate the difference in the
ability
of adhesion to the basement membrane and their biological activity between the normal gastrocyte and gastric cancer cells. Then, The role of basement membrane in tumor invasion was assessed by the evaluation of the invasion of gastric cancer
cells
of
different histological differentiation.
Gastrocytes were isolated from the surgically resected specimen by treating with collagenase and deoxyribonuclease. Normal gastrocytes were maintained viable up to 24 hours after isolation. The isolated cells were confirmed as gastric epithelial
cells
by indirect immunofluorescence staining for cytokeratin. Although the adhesion of normal gastrocyte was lower than that of gastric cancer cells, gastrocyte was bound bo laminin and type Ⅳ collagen. The substrates were shown to play an important
role
for the differentiation and regeneration of gastrocytes also. In the invasion assay using transwell cell culture chamber coated with matrigel, laminin and type Ⅳ collagen enhanced tumor cell invasion (p<0.05). However, there was no difference
according
to histological differentiation.
In conclusion, it can be suggested that laminin and type Ⅳ collagen must be important for the biological activity of normal gastrocyte and metastasis of gastric cancer cell. Type Ⅳcollagen enhances the invasion of gastric cancer cell more than
laminin
does.
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KEYWORD
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